Table 3.
T-cell responses to recombinant A. marginale T4SS proteins in Holstein cattle with different MHC class II haplotypes
| Antigena | Proliferation of T cells from animalb: |
|||||
|---|---|---|---|---|---|---|
| 35113 (11/22) | 35141 (22/24) | 35160 (3/16) | 35280 (16/27) | 35287 (16/22) | 583 (8/23) | |
| OM | ||||||
| 1 | 736.5 ± 5.7 | 21.9 ± 4.2 | 250.1 ± 13.1 | 50.2 ± 1.8 | 259.4 ± 9.2 | 220.0 ± 13.4 |
| 10 | 941.1 ± 52.8 | 36.7 ± 9.7 | 252.2 ± 9.2 | 44.0 ± 2.1 | 251.1 ± 9.2 | 312.1 ± 2.1 |
| URBC | ||||||
| 1 | 0.2 ± 0.1 | 0.8 ± 0.3 | 0.6 ± 0.1 | 0.3 ± 0.1 | 0.6 ± 0.2 | 0.4 ± 0.3 |
| 10 | 0.9 ± 0.7 | 1.6 ± 0.6 | 0.3 ± 0.1 | 0.8 ± 0.4 | 2.0 ± 2.6 | 0.9 ± 0.9 |
| MSA1 | ||||||
| 1 | 1 ± 1.3 | 0.9 ± 0.1 | 0.9 ± 0.1 | 0.4 ± 0.3 | 0.3 ± 0.2 | 0.1 ± 0.0 |
| 10 | 0.3 ± 0.1 | 0.8 ± 0.4 | 1.4 ± 0.2 | 0.4 ± 0.1 | 0.9 ± 0.5 | 0.1 ± 0.0 |
| VirB2 | ||||||
| 1 | 0.4 ± 0.1 | 2.0 ± 0.2 | 191.7 ± 33.6 | 9.4 ± 4.4 | 62.7 ± 17.1 | 3.4 ± 0.5 |
| 10 | 1.5 ± 0.3 | 2.8 ± 0.2 | 46.7 ± 2.2 | 3.3 ± 1.4 | 155.0 ± 2.5 | 7.9 ± 2.0 |
| VirB4-1 | ||||||
| 1 | 1.6 ± 0.7 | 0.5 ± 0.1 | 0.1 ± 0.0 | 0.2 ± 0.0 | 1.0 ± 0.3 | 1.0 ± 0.2 |
| 10 | 1.0 ± 0.5 | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.7 ± 0.2 | 2.6 ± 1.0 |
| VirB4-2 | ||||||
| 1 | 1.3 ± 0.1 | 0.3 ± 0.0 | 0.2 ± 0.2 | 0.2 ± 0.1 | 2.4 ± 1.9 | 1.3 ± 1.1 |
| 10 | 1.1±.1 | 0.1 ± 0.0 | 0.2 ± 0.2 | 0.1 ± 0.0 | 22.8 ± 19.6 | 1.8 ± 0.0 |
| VirB6-1 F1 | ||||||
| 1 | 3.0 ± 0.7 | 2.6 ± 0.7 | 8.2 ± 3.2 | 0.9 ± 0.3 | 0.9 ± 0.3 | 1.5 ± 1.1 |
| 10 | 0.7 ± 0.2 | 2.1 ± 0.4 | 78.4 ± 4.7 | 1.0 ± 0.2 | 1.5 ± 0.8 | 0.8 ± 0.1 |
| VirB7 | ||||||
| 1 | 0.5 ± 0.2 | 0.5 ± 0.1 | 0.7 ± 0.1 | 0.8 ± 0.1 | 1.2 ± 1.0 | 0.7 ± 0.1 |
| 10 | 2.0 ± 0.4 | 1.1 ± 0.3 | 2.2 ± 0.4 | 2.3 ± 2.1 | 1.8 ± 0.7 | 2.0 ± 0.8 |
| VirB8-2 | ||||||
| 1 | 1.3 ± 0.1 | 0.2 ± 0.0 | 0.7 ± 0.9 | 0.0 ± 0.0 | 0.9 ± 0.1 | 1.6 ± 0.5 |
| 10 | 0.9 ± 0.1 | 0.1 ± 0.0 | 0.1 ± 0.1 | 0.0 ± 0.0 | 1.2 ± 0.6 | 1.7 ± 0.6 |
| VirB9-1 | ||||||
| 1 | 385.7 ± 36.5 | 22.5 ± 4.2 | 0.7 ± 0.1 | 46.8 ± 11.2 | 264.7 ± 10.6 | 0.1 ± 0.1 |
| 10 | 779.3 ± 71.4 | 8.0 ± 4.6 | 0.9 ± 0.2 | 103.7 ± 16.4 | 239.8 ± 11.5 | 0.1 ± 0.0 |
| VirB9-2 | ||||||
| 1 | 139.2 ± 11.7 | 35.3 ± 16.0 | 0.8 ± 0.1 | 8.0 ± 2.8 | 32.8 ± 4.1 | 25.9 ± 4.4 |
| 10 | 201.5 ± 27.1 | 32.8 ± 0.7 | 1.7 ± 0.8 | 11.5 ± 2.1 | 109.6 ± 4.3 | 303.7 ± 19.5 |
| VirB10 | ||||||
| 1 | 35.5 ± 3.6 | 3.7 ± 0.5 | 90.6 ± 9.4 | 0.1 ± 0.0 | 96.2 ± 11.6 | 6.7 ± 2.7 |
| 10 | 381.2 ± 39.0 | 5.8 ± 0.3 | 126.2 ± 5.6 | 0.3 ± 0.0 | 162.5 ± 2.3 | 72.9 ± 8.6 |
| VirB11 | ||||||
| 1 | 1.8 ± 0.1 | 0.6 ± 0.1 | 0.1 ± 0.0 | 0.1 ± 0.0 | 1.0 ± 0.4 | 0.0 ± 0.0 |
| 10 | 3.2 ± 1.4 | 0.7 ± 0.2 | 0.0 ± 0.0 | 0.1 ± 0.0 | 0.8 ± 0.2 | 0.1 ± 0.0 |
| VirD4 | ||||||
| 1 | 0.4 ± 0.1 | 2.7 ± 0.4 | 30.4 ± 6.2 | 0.3 ± 0.1 | 0.4 ± 0.1 | 2.8 ± 1.2 |
| 10 | 0.5 ± 0.0 | 1.7 ± 0.1 | 99.7 ± 6.4 | 0.5 ± 0.1 | 0.5 ± 0.0 | 6.3 ± 4.0 |
a
Recombinant T4SS proteins were used at 1 and 10 μg/ml in the T-cell assays, as indicated. A. marginale OM and URBC were positive and negative controls, respectively.
b
A stimulation index (SI) was calculated as the mean cpm of a 2-week CD4 T-cell line to an individual protein/the mean cpm to medium. Results in boldface indicate significantly greater T-cell responses to a T4SS protein compared to the response to the same concentration of MSA1, where P was <0.05 using the Dunnett's test. Animal numbers (DRB3 RFLP types) are indicated in the column subheadings.