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Liquid CAS assays for ABU E. coli strain 83972 wild-type and siderophore biosynthetic deletion mutants in MM9 medium at pH 5.5 and pH 7.0. Results are the means of biological triplicates plus standard deviations. Bar graph shows percent siderophore units, calculated as [(Ar − As)/Ar] × 100, where Ar is the absorbance of MM9/CAS solution and As is the sample absorbance. The quadruple mutant E. coli 83972 EASY had negligible siderophore activity at both pHs.
