Fig 2.

Rabbit antienolase serum recognizes both recombinant and native enolase. (A) Immunoblot showing recognition of the recombinant enolase (3 μg/lane). Lane 1, preimmunization rabbit serum does not recognize recombinant enolase; lanes 2 to 5, results for rabbit antienolase serum obtained at intervals in the immunization period. (B) Immunoblot. Preimmunization rabbit serum (1/100) does not react to whole Borrelia lysate. (C) Rabbit antienolase serum recognized native enolase in B. burgdorferi whole lysate. Molecular mass markers are shown at the left of each panel.