Fig 4.

Digestion of whole B. burgdorferi and recombinant enolase by proteinase K. (A) B. burgdorferi (10⁸) were treated for 1 h at room temperature (23°C) with proteinase K (PK) at the concentrations indicated and analyzed by SDS-PAGE and Western blot to determine whether portions of enolase were surface exposed. Individual gel lanes received approximately 3 × 10⁷ control or PK-treated spirochetes. (B) Recombinant enolase (4 μg) was incubated for 1 h at room temperature in PBS–5 mM MgCl₂ with or without PK (250 μg/ml), separated by SDS-PAGE, and stained with Coomassie brilliant blue. The results shown are from a representative experiment.