Fig 6.

p300 is involved in the PNR-mediated stimulation of p53 in HeLa cells. (A) Two different shRNA plasmids targeting p300 inhibited the PNR-mediated stimulation of p53RE-FLuc expression. The RLU in the PNR+shRNA-Con sample was normalized to 100%. (B) shRNA targeting p300 inhibits the PNR-mediated stimulation of p53 acetylation and accumulation. The indicated proteins were measured by immunoblotting. The numbers below the p300 band represent the levels of p300 protein normalized to the p300 level in sample 1. shRNA-Con, pLKO.1 empty lentiviral control.