Fig 8.

PNR enhances formation of a complex consisting of p53 and p300 with or without actinomycin D treatment in HeLa cells. (A) PNR enhances p300's binding to total p53 and Ac-p53. Anti-p300 antibody coimmunoprecipitated p53. Both p53 and Ac-p53 (K373 + 382) were measured by immunoblotting. Upper arrow, Ac-p53; lower arrow, rabbit IgG heavy chain. (B) PNR boosts actinomycin D-enhanced association between p53 and p300. One day after cotransfection of the indicated plasmids, cells were treated with 10 nM actinomycin D for 24 h and then processed as described for panel A.