Fig 4.

GATA-1 occupies genes encoding ATG8 homologs in primary human erythroblasts and G1E-ER-GATA-1 cells. (A) ChIP-seq profiles of GATA-1 occupancy at loci encoding mammalian ATG8 homologs in primary human erythroblasts. ATG8 homolog genes are arranged in a 5′ → 3′ orientation. (B) ChIP analysis of ER-GATA-1 occupancy at mammalian Atg8 homologs in untreated and β-estradiol-treated (24 h) G1E-ER-GATA-1 cells at sites corresponding to the GATA-1 occupancy sites in the primary human erythroblasts (mean ± standard error; 3 independent experiments). The results obtained with the preimmune antibody (PI) are depicted by the left bar for each condition. pro, promoter.