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. 2012 Jan;32(1):226–239. doi: 10.1128/MCB.06166-11

Fig 9.

Fig 9

FoxO3-dependent mechanism of GATA-1-mediated autophagy gene regulation. (A) FoxO3 knockdown strategy in G1E-ER-GATA-1 cells. (B and C) Real-time RT-PCR analysis of gene expression in control and FoxO3-knockdown G1E-ER-GATA-1 cells (mean ± standard error; 4 independent experiments; ∗, P < 0.05; ∗∗, P < 0.01). Values were normalized to 18S rRNA expression levels. (D) Semiquantitative Western blot analysis of LC3-I, LC3-II, ER-GATA-1, and tubulin in control and FoxO3-knockdown G1E-ER-GATA-1 cells. (E) Semiquantitative Western blot analysis of GABARAP-I, GABARAP-II, ER-GATA-1, and tubulin in control and FoxO3-knockdown G1E-ER-GATA-1 cells. A representative image is shown from two independent experiments. (F) Real-time RT-PCR analysis of FoxO3 transcript levels in G1E-ER-GATA-1 cells following ER-GATA-1 activation for various times (mean ± standard error; 3 independent experiments; ∗∗, P < 0.01). Values were normalized to 18S rRNA expression levels. (G) ChIP-seq profile of GATA-1 occupancy at FOXO3 in primary human erythroblasts. (H) Expression level of FOXO3 during primary human erythroblast differentiation from Human Erythroblast Maturation Database.