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. 2012 Jan;32(1):126–138. doi: 10.1128/MCB.06077-11

Fig 6.

Fig 6

SMN deficiency decreases nuclear levels of SmB protein in SMA motor neurons. (A) Immunohistochemistry analysis with anti-SmB antibodies of a spinal cord section from control (Smn+/+; SMN2+/+; SMNΔ7+/+) mouse at postnatal day 10. Higher-magnification images of dorsal and ventral horn regions are shown on the right. Note the strong ubiquitous nuclear SmB immunoreactivity as well as detectable staining of the cytoplasm in large ventral horn cells that are likely motor neurons. Bars, 10 μm. (B) Immunohistochemistry analysis with anti-SmB antibodies of a spinal cord section from Δ7 SMA (Smn−/−; SMN2+/+; SMNΔ7+/+) mouse at postnatal day 10. Higher-magnification images of dorsal and ventral horn regions are shown on the right. There is a reduction in SmB staining of large ventral horn cells from SMA mice compared to control mice. Bars, 10 μm. (C) Analysis by double-label immunofluorescence and confocal microscopy of spinal cord sections from control (Smn+/+; SMN2+/+; SMNΔ7+/+) and Δ7 SMA (Smn−/−; SMN2+/+; SMNΔ7+/+) mice at postnatal day 10 with anti-SmB and anti-Hb9 antibodies. Arrowheads point to SMA motor neurons with strongly reduced SmB levels. Bars, 10 μm. (D) Quantification of SmB and Hb9 expression in the nucleus of motor neurons. Each data point represents the average fluorescence intensity per unit area in individual Hb9+ nuclei from control (n=72) and SMA (n=42) motor neurons measured from confocal images as in panel C. Means and SEM are shown (***, P < 0.001).