Fig 1.

Domain organizations of Cdc13s and the role of Candida Cdc13 in telomere regulation. (A) The different domain organizations of Cdc13 homologues from Saccharomyces and Candida species are illustrated. The OB1 and RD domains of Saccharomyces Cdc13 have been shown to interact with Pol1 and Est1, respectively. (B) The wild type (BWP17) and strains heterozygous for CDC13 (with or without a C-terminal TAP tag) were passaged in YPD+uri and analyzed for telomere lengths. For each heterozygous CDC13^+/− clone (with or without TAP tag), telomeres were examined after 3, 6, and 9 streaks on plates. (C) Chromosomal DNAs from the indicated strains were digested with AluI and NlaIII and then subjected to two-dimensional gel electrophoresis and Southern blotting to assess the levels of linear and circular telomeric DNAs (marked by an arrow). (D) (Top) The expression of TAP-tagged Cdc13 protein in extracts derived from the untagged and tagged strains were analyzed by Western blotting using antibodies directed against protein A. The positions of CaCdc13-TAP and two cross-reacting proteins are indicated by an arrow and two asterisks, respectively. (Bottom) Strains with or without TAP-tagged Cdc13 were subjected to ChIP analysis using IgG-Sepharose. The input (0.13, 0.64, and 3.2%) and precipitated DNAs (100%) were spotted on nylon filters and probed with labeled C. albicans telomere repeats.