Fig 3.

The effect of telomere DNA length on CtCdc13-DNA interaction. (A) The sequences of oligonucleotides used as probes or competitors in this series of assays are listed. (B) EMSAs were performed using increasing concentrations of CtCdc13 (0, 10, 20, and 40 nM) and either TEL-GX1 or TEL-GX2 as the probe. (C) Competition EMSAs were performed using 10 nM CtCdc13 and 7.5 nM labeled TEL-GX2. Increasing concentrations of four different competitor oligonucleotides were added, and the levels of DNA-protein complexes were quantified and plotted. Data are from three independent experiments.