Fig 7.

The effects of OB4 mutations on DNA binding by CtCdc13. (A) The locations of the OB4 mutations in the full-length CtCdc13 are illustrated. (B) (Left) Purified SUMO-CtCdc13 mutant proteins bearing substitutions in the OB4 domain were analyzed by SDS-PAGE and Coomassie staining. (Right) Serial dilutions of the purified protein preparations were analyzed by SDS-PAGE and Western blotting using anti-His antibodies. (C) EMSAs were performed using increasing concentrations (116, 232, and 464 nM) of SUMO-fused wild-type and mutated CtCdc13s bearing amino acid replacements in the OB4 domain and the TEL-GX1.5B probe (7.5 nM). FL, full length.