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DNA binding by the CtCdc13-L45 mutant. (A) The purified CtCdc13 and CtCdc13-L45 (without the SUMO tag) were analyzed by SDS-PAGE and Coomassie staining. (B) The indicated concentrations of wild-type and L45 mutant proteins were tested for binding to the TEL-GX2 probe. (C) The SUMO-CtCdc13-L45 fusion protein was incubated in the absence or presence of ULP1 protease, and then tested for binding to the TEL-GX2 probe. WT, wild type.
