Fig 4.

Mink1 is required for the localized accumulation of Pk in the plasma membrane. Venus-PRICKLE1 mRNA (500 pg) and membrane-bound RFP (mb-RFP) mRNA (100 pg) were injected in dorsal blastomeres of 4-cell Xenopus embryos. The cellular localization of Pk was monitored in dorsal explants at stage 11 using a confocal microscope under a 40× oil immersion objective. The plasma membrane is highlighted using membrane-bound RFP (mb-RFP). (A to C) During convergence and extension, PRICKLE1 localizes at the membrane and accumulates in plasma membrane subdomains (yellow arrows). (D to F) Downregulation of xMink1 using 13 ng of MO-xMink1 leads to an absence of PRICKLE1 puncta. PRICKLE1-T370A (G to I) or PRICKLE1ΔLIM2 (J to L) does not localize efficiently to puncta. Images shown are representative of two (PRICKLEΔLIM2) or four (PRICKLE1, xMink1-MO, and PRICKLE1-T370A) independent experiments. Scale bars represent 50 μm.