Table 1.

List of proteins found in PRICKLE1 and PRICKLE2 complexes by LC-MS/MS^a

Gene no.	Gene name	Protein name	Results of LC-MS/MS for:
			FLAG-PRICKLE1				FLAG-PRICKLE2
			No. of unique peptides	Total no. of peptides	% Coverage	No. of pulldowns	No. of unique peptides	Total no. of peptides	% Coverage	No. of pulldowns
144165	PRICKLE1		57	2,031		2
166336	PRICKLE2						22	649	30.2	2
81839	VANGL1	Vang-like 1 (van Gogh, Drosophila)	3	3	7.6	1	2	2	5.3	1
57216	VANGL2	Vang-like 2 (van Gogh, Drosophila)	1	1	2.3	1
1460	CSNK2B	Casein kinase 2, beta subunit	7	24	32.1	1	4	7	40	2
1457	CSNK2A1	Casein kinase 2, alpha 1 subunit	18	85	46.8	1	13	31	34	2
1459	CSNK2A2	Casein kinase 2, alpha prime subunit	8	34	38.6	1	9	29	36.9	2
55914	ERBB2IP	Erbb2 interacting protein	15	25	16.7	2	17	31	18.3	2
613	BCR	Breakpoint cluster region	59	694	49.3	2	38	248	32.2	2
50488	MINK1	Misshapen-like kinase 1	17	25	18.2	2	6	12	7.1	2
85461	TANC1	Tetratricopeptide repeat, ankyrin repeat, and coiled-coil containing 1	27	46	22.7	2	3	3	2.5	2
26115	TANC2	Tetratricopeptide repeat, ankyrin repeat, and coiled-coil containing 2	18	38	13.8	2	12	18	9.3	2
400745	SH2D5	SH2 domain containing 5	13	44	52	2	6	18	26.8	2
253260	RICTOR	RPTOR independent companion of MTOR, complex 2	8	18	9	2	7	14	6.3	2

^aPeptides from trypsin-digested PRICKLE1- and PRICKLE2-associated protein complexes were analyzed by LC-MS/MS. The peptides and resulting proteins were identified using an integrated version of SEQUEST and analyzed with the Peptide and Protein Prophet software suite (ISB, Seattle, WA). The unique peptides column represents the total number of unique peptides successfully identified. Total peptides corresponds to the total number of peptides matching a given protein identification. Percent coverage is the percentage of the protein sequence covered by the peptide sequences identified. Two independent experiments were performed for both PRICKLE1 and PRICKLE2, and the frequency with which a protein was identified is designated the number of pulldowns.