Table 1.
Gene no. | Gene name | Protein name | Results of LC-MS/MS for: |
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---|---|---|---|---|---|---|---|---|---|---|
FLAG-PRICKLE1 |
FLAG-PRICKLE2 |
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No. of unique peptides | Total no. of peptides | % Coverage | No. of pulldowns | No. of unique peptides | Total no. of peptides | % Coverage | No. of pulldowns | |||
144165 | PRICKLE1 | 57 | 2,031 | 2 | ||||||
166336 | PRICKLE2 | 22 | 649 | 30.2 | 2 | |||||
81839 | VANGL1 | Vang-like 1 (van Gogh, Drosophila) | 3 | 3 | 7.6 | 1 | 2 | 2 | 5.3 | 1 |
57216 | VANGL2 | Vang-like 2 (van Gogh, Drosophila) | 1 | 1 | 2.3 | 1 | ||||
1460 | CSNK2B | Casein kinase 2, beta subunit | 7 | 24 | 32.1 | 1 | 4 | 7 | 40 | 2 |
1457 | CSNK2A1 | Casein kinase 2, alpha 1 subunit | 18 | 85 | 46.8 | 1 | 13 | 31 | 34 | 2 |
1459 | CSNK2A2 | Casein kinase 2, alpha prime subunit | 8 | 34 | 38.6 | 1 | 9 | 29 | 36.9 | 2 |
55914 | ERBB2IP | Erbb2 interacting protein | 15 | 25 | 16.7 | 2 | 17 | 31 | 18.3 | 2 |
613 | BCR | Breakpoint cluster region | 59 | 694 | 49.3 | 2 | 38 | 248 | 32.2 | 2 |
50488 | MINK1 | Misshapen-like kinase 1 | 17 | 25 | 18.2 | 2 | 6 | 12 | 7.1 | 2 |
85461 | TANC1 | Tetratricopeptide repeat, ankyrin repeat, and coiled-coil containing 1 | 27 | 46 | 22.7 | 2 | 3 | 3 | 2.5 | 2 |
26115 | TANC2 | Tetratricopeptide repeat, ankyrin repeat, and coiled-coil containing 2 | 18 | 38 | 13.8 | 2 | 12 | 18 | 9.3 | 2 |
400745 | SH2D5 | SH2 domain containing 5 | 13 | 44 | 52 | 2 | 6 | 18 | 26.8 | 2 |
253260 | RICTOR | RPTOR independent companion of MTOR, complex 2 | 8 | 18 | 9 | 2 | 7 | 14 | 6.3 | 2 |
Peptides from trypsin-digested PRICKLE1- and PRICKLE2-associated protein complexes were analyzed by LC-MS/MS. The peptides and resulting proteins were identified using an integrated version of SEQUEST and analyzed with the Peptide and Protein Prophet software suite (ISB, Seattle, WA). The unique peptides column represents the total number of unique peptides successfully identified. Total peptides corresponds to the total number of peptides matching a given protein identification. Percent coverage is the percentage of the protein sequence covered by the peptide sequences identified. Two independent experiments were performed for both PRICKLE1 and PRICKLE2, and the frequency with which a protein was identified is designated the number of pulldowns.