Fig 4.

Correlation between IgH transcription and accumulation of nonfunctional pre-mRNAs. B cell populations were sorted from heterozygous IgH^frVκ/wt mice as described in the legend of Fig. 3. (A to C) NF/F IgH pre-mRNA ratios were determined for cDNAs by qPCR using allele-specific primers (sets A and B in Fig. 1A) after normalization to the NF/F ratio obtained by using DNA from IgH^frVκ/wt mice (NF/F_DNA, set to 1). Dotted lines corresponds to the mean of NF/F values obtained for RNAPII-bound fractions (NF/F ratio=0.68) (Fig. 2B). This value was used as a threshold reference and reflects the RNAPII loading on both VDJ-rearranged IgH alleles in B cell populations harboring either biallelic (VDJ⁺/VDJ⁻) or monoallelic (VDJ⁺/DJ) VDJ rearrangements. (D) Relative levels of functional IgH pre-mRNAs (IgH^wt) in B and plasma cells were determined by using allele-specific primers (set A in Fig. 1A) after normalization to β-actin gene transcripts. Values obtained for resting B cells were set to 1. Results from 5 to 7 independent cell sorting experiments are shown. (∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001).