Table 1.
Primer sequences, reaction conditions, and product lengths
| Target gene | Primer | Sequence (5′–3′) | Annealing temp (°C) | Elongation time | Amplified fragment size (bp) |
|---|---|---|---|---|---|
| SYCP3 | MSYCP35′Kpn1 | GACGGTACCATGCTTCGAGGGTGTGGG | 52 | 1 min | 900 |
| MSYCP3r | TTGACACAATCGTGGAGAGAA | ||||
| Stra8 | Stra8 for | TCACAGCCTCAAAGTGGCAGG | 56 | 30 s | 444 |
| Stra8 rev | GCAACAGAGTGGAGGAGGAGT | ||||
| HANP1/H1T2 | Hanp1-H1T2-for | GCTGGCTACTTCAGGGTCT | 50 | 1 min | 800 |
| Hanp1-H1T2-rev | TGTATGCTGGGAGCGTTG | ||||
| MIS | MMISf | TTGGTGCTAACCGTGGACTT | 56 | 30 s | 315 |
| MMISr | GCAGAGCACGAACCAAGCGA | ||||
| Ins13 | mIns13f | TACTGATGCTCCTGGCTCTGG | 56 | 30 s | 543 |
| mIns13r | TTAGACTGTTTGGGACACAGG | ||||
| Odf2 | 138NCEcoRI | GGAATTCATGTCCGCCTCATCCTCAGGC | 59 | 1 min | 822/677 |
| DH1rev | CAGCTTCCCGATGGTATCCTTCAAG | ||||
| Odf1 | Odf1-N | GAGCTCAAGCTTTGGCCGCACTGAGTTGTC | 52 | 1 min | 800 |
| Odf1-C | CCGCGGTACCCAAGATCATCTTCCTACA |