Table 3.
GN2 plate substrate | Respiration of E. coli O157:H7 isolatesa |
||||||
---|---|---|---|---|---|---|---|
Spinach RM6067 | Environmental |
Spinach clinical |
|||||
RM6101 | RM6102 | RM6103 | RM6149 | RM9998 | RM6069 | ||
Dextrin | 178 | 111 | 150 | 173 | 108 | 283 | 275 |
l-Rhamnose | 61 | 20 | 48 | 92 | 23 | 197 | 192 |
Methyl pyruvate | 204 | 198 | 211 | 282 | 162 | 416 | 477 |
Mono-methylsuccinate | 176 | 217 | 242 | 226 | 134 | 486 | 442 |
d-Glucuronic acid | 241 | 350 | 381 | 394 | 310 | 585 | 520 |
d,l-Lactic acid | 152 | 159 | 197 | 367 | 192 | 539 | 433 |
Succinic acid | 209 | 206 | 288 | 251 | 200 | 413 | 395 |
d-Alanine | 6 | 3 | 7 | 190 | 7 | 318 | 214 |
l-Alanine | 82 | 94 | 115 | 262 | 103 | 401 | 330 |
l-Alanyl-glycine | 49 | 21 | 72 | 114 | 62 | 292 | 227 |
Glycyl-l-aspartic acid | 51 | 17 | 22 | 42 | 21 | 251 | 265 |
l-Serine | 93 | 14 | 40 | 48 | 21 | 333 | 465 |
l-Threonine | 13 | 13 | 25 | 20 | 14 | 115 | 125 |
Glycerol | 185 | 143 | 243 | 241 | 152 | 458 | 433 |
d,l-α-Glycerolphosphate | 147 | 134 | 199 | 219 | 119 | 443 | 449 |
Values are a unitless measure of respiration based on the reduction of tetrazolium dye. Values are optical density units reflecting color formation due to oxidation of the compounds by bacterial cells. Optical density for each substrate was normalized to that in control wells (no substrate).