Fig 3.

Exchange of ScHSF1 or K7HSF1 alleles has no apparent effects on Hsf1p-mediated stress responses. (A) Mutation points in the K7HSF1 allele revealed by whole-genome sequencing of K7 (http://nribf1.nrib.go.jp/SYGD). White circles, arrows, and the asterisk indicate nonsynonymous SNPs, other SNPs, and an adenine-nucleotide insertion, respectively. (B) β-Galactosidase activities of the HSE-pCYC1-lacZ reporter in X2180 Δhsf1 AUR1::AUR1-C-HSE-pCYC1-lacZ and K701 Δhsf1 AUR1::AUR1-C-HSE-pCYC1-lacZ background strains expressing ScHSF1 or K7HSF1 under nonstress (■) and 8% ethanol stress (□) conditions. The data represent average values ± the SD of three or more independent experiments. (C) Phosphorylation states of Hsf1p in X2180 Δhsf1 and K701 Δhsf1 background strains expressing ScHSF1 or K7HSF1 under nonstress and 8% ethanol stress conditions. The same symbols as in Fig. 2 are used to show the phosphorylation states of Hsf1p, whose respective positions were determined by comparison with the control samples analyzed in the same gel. The arrow indicates the direction of electrophoretic migration.