Table 2.
GFC and GFD qPCR assay performance characteristics based on 11 standard curves separately run over a 6-month period
| Assay | Range of values for: |
||||
|---|---|---|---|---|---|
| Slope | Intercept | Efficiencya | r2 | ROQb (no. of copies) | |
| GFC | −4.06, −3.34 | 34.55, 39.54 | 0.88, 1.00 | 0.990, 0.996 | 20-2 × 105 |
| GFD | −3.51 −3.02 | 32.52, 38.26 | 0.96, 1.07 | 0.993, 0.999 | 200-2 × 105 |
a
Defined as 10(1/−slope)/2.
b
Range of quantification: plasmid concentration range that remained linear to CT values (r2 >0.98) in all 11 standard curves over the 6-month period.