Fig 6.

Yeast Las1 is required for large rRNA pre-rRNA processing. Pre-rRNA processing was analyzed by Northern blotting. Total RNA was extracted from pGAL::HA-las1 cells grown to mid-log phase in YPGSR at 30°C, washed in water, and transferred to YPD for up to 12 h. The RNA was separated on a denaturing agarose-formaldehyde gel, transferred to a nylon membrane, and hybridized with a set of oligonucleotide probes specific to mature and precursor rRNAs (see schematics at the right). Probes used are indicated in each panel and on the schematics. At the bottom of panel III is a longer exposure for 7S. Fuji FLA7000 quantitations are summarized in cartoons, and the 27S/7S and 25S/18S ratios are provided (see Table S2 in the supplemental material for details).