Fig 4.

Depletion of Siah2 leads to stabilization of TIN2. (A) RT-PCR analysis of RNA isolated from HeLaI.2.11 cells transfected with GFP or Siah2 siRNA. PCR was performed with primers specific for Siah2 or GAPDH in reactions without cDNA (−) or with cDNA (+) prepared without (−) or with (+) RT. Siah2 DNA levels relative to GAPDH and normalized to the GFP control are indicated below the upper gel. (B) Immunoblot analysis of extracts from HeLaI.2.11 cells transfected with Siah2 or GFP siRNA. The blot was stained with Amido black (bottom panel), and proteins were detected by probing with anti-TIN2 701, anti-TRF1 415, anti-TRF2, anti-TPP1 911, or anti-α-tubulin. Protein levels relative to α-tubulin and normalized to the vector control are indicated between the blots.