Fig 7.

Mitochondrial morphology and proteins related to mitochondrial dynamics in the skeletal muscles of obese mice. (A) Mitochondrial morphology imaged by transmission electron microscopy. (B) Distribution of mitochondrial areas and lengths in the gastrocnemius muscles of ob/ob and control mice. Each scale bar is 500 nm. Also shown are immunoblot analyses of the protein contents of the mitochondrial fraction of the muscle tissues of ob/ob and control mice at the age of 13 weeks (C), mice fed HF and LF diets for 10 weeks (D), and mice fed HF and LF diets for 16 weeks (E). Samples from representative animals are shown in the Western blot assay, with each lane representing one animal. The intensities of the bands, quantified densitometrically relative to that of the respective controls, are shown with the sample number in parentheses. *, P < 0.05; **, P < 0.01. Porin was used as a loading control for mitochondrial extracts.