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. 2012 Jan;32(2):490–500. doi: 10.1128/MCB.06361-11

Fig 3.

Fig 3

Restoration of Akt1 expression reduces osteogenic differentiation. (A to C) Experimental scheme: MEFs from WT and Akt1 KO mice were infected with recombinant lentiviruses (LV) encoding mouse Akt1, mouse Akt2, or EGFP and were incubated in OM plus 200 ng/ml BMP2 for up to 7 days. (B) Immunoblots of whole-cell protein lysates for Akt1, Akt2, total Akt, Runx2, and α-tubulin. (C) AP staining on day 5 and Alizarin red on day 7. (D to F) Experimental scheme: C3H10T1/2 cells were infected with Ad-shAkt1 or Ad-βgal (Con), followed by infection with LV-Akt1, LV-Akt2, or LV-EGFP, and incubation in OM plus 200 ng/ml BMP2. (E) Immunoblots of whole-cell protein lysates for Akt1, Akt2, total Akt, Runx2, and α-tubulin on day 5. (F) AP staining on day 3.