Fig 6.

Coculture of bone marrow macrophages with Akt1-deficient osteoblasts reduces osteoclast formation. (A) Mouse bone marrow macrophages were incubated with MEFs from wild type (WT) or Akt1 knockout (KO) mice in osteogenic medium plus BMP2 for 7 days. (B) TRAP staining to assess formation of multinucleated osteoclasts at day 7. (C) Percentage of TRAP-positive cells at day 7 expressed as area per microscopic field (×200 magnification; mean ± SD of results of six fields; ∗, P < 0.005 versus WT). (D) Comparison of the number of TRAP-positive osteoclasts per microscopic field with >4 nuclei on day 7 (×200 magnification; mean ± SD of results of six fields; ∗∗, P < 0.001 versus WT).