Fig 6.

Meiotic progression, Sum1 phosphorylation, and NDT80 expression in cdc7-as diploids containing various alleles of SUM1. The cdc7-as sum1Δ diploid, NH788-XC, containing two copies of either pRS306, SUM1, sum1-12A, or sum1-c4i, was transferred to Spo medium and analyzed at different times. (A) Meiotic progression was monitored by DAPI staining of cells sporulated in the absence or presence of 15 μM PP1. (B) Immunoblots of cdc7-as SUM1, cdc7-as sum1-c4i, and cdc7-as sum1-12A strain time courses probed with anti-Sum1 and anti-Ndt80 antibodies as indicated. (C) Side-by-side comparison of Sum1 and Ndt80 mobilities in the cdc7-as SUM1, cdc7-as sum1-c4i, and cdc7-as sum1-12A diploids from the 6-h time point.