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. 2012 Jan;32(2):541–557. doi: 10.1128/MCB.06032-11

Fig 7.

Fig 7

Chromatin immunoprecipitation of Sum1-3Flag at the NDT80 and SMK1 promoters in the absence or presence of Cdc7 kinase. ChIP was performed using anti-Flag antibodies on chromatin isolated from cdc7-as ndt80Δ diploids carrying either Sum1-3Flag (NH1080) or untagged Sum1 (NH932) under three conditions: 0 or 8 h after transfer to Spo medium in the absence or presence of 15 μM PP1. Three independent experiments were performed for each strain, and the standard deviations are indicated by error bars. (A) q-PCR results from Sum1-3Flag diploid using primers flanking the MSEs in the NDT80 promoter normalized to the negative-control primers. (B) q-PCR results from the untagged diploid using primers flanking the MSEs in the NDT80 promoter normalized to the negative-control primers. Note the change in scale. (C) q-PCR results from Sum1-3Flag diploid using primers flanking the MSEs in the SMK1 promoter normalized to the negative-control primers.