Fig 6.

Conservation of repression activity by the N termini of human PUFs PUM1 and PUM2. (A) Comparison of human PUM1 and PUM2 to Drosophila Pumilio. The percentage of identical amino acids between segments is indicated. (B) Percent repression by the N terminus of each protein, fused to MS2 coat protein, was determined relative to control MS2 alone. Test proteins include the N termini of Drosophila Pumilio (DmPum-N) and human PUM1 (HsPUM1-N) and PUM2 (HsPUM2-N). (C) Percent repression by MS2-tethered regions of human PUM1 corresponding to Pumilio repression domains (HsPUM1-1, HsPUM1-2, and HsPUM1-3). All assays used RnMS2 reporter and the FF control. Western blot analysis confirmed expression of V5 epitope-tagged proteins. Data are provided for panels B and C in Tables S16 and S17 in the supplemental material, respectively.