Fig 4.

KDM2A interacts with linker DNA in vivo. (A) Nuclei were partially digested with micrococcal nuclease to produce polynucleosomes that contain linker DNA. Chromatin was separated by sucrose density gradient centrifugation. The gradient was fractionated from the top into 24 fractions. DNA was purified from each fraction and resolved by agarose gel electrophoresis (top panel), and proteins from each fraction were visualized by Western blotting (bottom panels). DNA and protein analyses include an unfractionated input sample representing 10% of material loaded onto the sucrose gradient. (B) As above, except that native chromatin was digested to completion with micrococcal nuclease to produce mononucleosomes lacking linker DNA. (C) Fractionation of chromatin-free nuclear extract.