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Expression of wild-type RISP in KO cells reconstituted the levels of fully assembled CIII and CIV. (A) BNGE and Western blot of KO fibroblasts transduced with GFP, wild-type (WT) RISP, or mutant (MUT) RISP lentivirus. WT RISP restored levels of fully assembled CI and increased levels of CIII and CIV in KO cells. (B) BNGE and in-gel activity stain for CI and CIV. (C) Steady-state levels of OXPHOS complex subunits by Western blotting.
