Fig 9.

Scavenging superoxide radicals increases CI and supercomplex stability. (A) BNGE and Western blot of cells treated with either NAC (10 mM) or MnTBAP (100 μM). (B) BNGE of control cells treated with antimycin A (20 nM) and the effects of different concentrations of either NAC or MnTBAP on supercomplex (SC) stability. (C) BNGE of control and RISP KO fibroblasts infected with lentivirus expressing mitochondrial SOD2. Antibodies used are indicated beside each panel. The levels of SOD2 were determined by SDS-PAGE. VDAC1 and Tim23 were used as protein loading controls.