Fig 6.

The N-terminal portion of ZfL2-1 ORF1p does not induce strand exchange between two duplexes. Strand exchange assays were performed with 1 μM Trx or the Trx fusion of the N-terminal portion of ZfL2-1 ORF1p (Trx-N-ORF1p) at 37°C. Schematic diagrams of the strand exchange assays (top) and the resulting autoradiogram (bottom) are shown. In the diagrams, oligonucleotides are indicated by blue and red lines. Their sequences are shown in Table 2. One strand of each initial duplex was labeled by ³²P at its 5′ end (asterisks). The duplex (co33-[³²P]o29) was incubated for the indicated times with a 50-fold excess of the single-stranded oligonucleotide co29 (left), the duplex co29-o29 (middle), or the duplex co29-o33 (right).