. 2012 Jan;32(2):458–469. doi: 10.1128/MCB.06162-11

Table 2.

Oligonucleotides used in the annealing, melting, and strand exchange assays^a

Name	Sequence (5′ to 3′)
co25	GCGAGTTGATGTTAGACTGTGTACT
o29	AAAAAGTACACAGTCTAACATCAACTCGC
co29	GCGAGTTGATGTTAGACTGTGTACTTTTT
co29^4m	GCGAGTTGACGTCAGACCGTGCACTTTTT
o29^3′	AGTACACAGTCTAACATCAACTCGCAAAA
o33	AAAAAGTACACAGTCTAACATCAACTCGCAAAA
co33	TTTTGCGAGTTGATGTTAGACTGTGTACTTTTT
o29^T4	AAAAAGTACACAGTCTAACATCAACTCGCTTTT
o29^3′T4	TTTTAGTACACAGTCTAACATCAACTCGCAAAA

Oligonucleotides are named for their length except for o29^T4 and o29^3′T4, which are 33 nucleotides long. “T4” indicates that four T's were added to one end, and “c” indicates the complementary strand. The nucleotides in co29^4m that form mismatches when hybridized to o29 are underlined.