Fig 3.

Comparison of HIV-2 neutralization titrations using different assay formats. (A) Neutralization of human CD4⁺ lymphocyte-grown HIV-2_7312A and HIV-2_ST stocks by four HIV-2-infected patient plasma samples in the human CD4⁺ T-cell multicycle infectivity assay. (B) Neutralization of human CD4⁺ T-cell-grown versus 293T-cell-grown HIV-2_7312A and HIV-2_ST by five HIV-2-infected patient plasma samples in the TZM-bl assay. (C) Summary analysis of reciprocal IC₅₀ neutralization shown in panels A and B. One-tailed paired t tests were performed, and P values are shown. Error bars were generated on the basis of at least three independent experiments. Dashed line indicates 50% reduction in virus infectivity.