Fig 5.
Absence of MPER neutralizing antibodies in serum from HIV-2-infected patients. (A) Construction of the HIV-1/HIV-2 chimeric virus BG1168.1-C1 containing the complete MPER from HIV-2ST. (B) Neutralization of HIV-1BG1168.1, HIV-2ST, and BG1168.1-C1 by anti-MPER antibodies 4E10 and 2F5. (C) Neutralization of HIV-1BG1168.1, HIV-2ST, and BG1168.1-C1 by 53 HIV-2-infected patient serum samples. Positive neutralization was defined as a reciprocal IC50 of at least 100. One HIV-2-infected serum sample was positive for neutralization of the BG1168.1-C1 chimera with a reciprocal IC50 titer of 252. The median reciprocal IC50 titer of the 53 serum specimens against HIV-2ST was 2.8 × 104.