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Potential N-linked glycosylation sites in the V4 loops of HIV-1 and HIV-2 strains. Mean values are shown as dashed lines. For HIV-1, the mean was 4.34, the median was 4, and the range was 2 to 7. For HIV-2, the mean was 2.48, the median was 2, and the range was 2 to 3. The HIV-1 V4 sequences exhibited a significantly higher level of glycosylation than the HIV-2 V4 sequences (P < 0.0001, Mann-Whitney test).
