Fig 4.

Heterotypic blockade of GII.4 VLP-Bi-HBGA interactions by anti-GII.4-2002-G6. (A) Increasing concentrations of anti-GII.4-2002-G6 antibody were incubated with additional GII.4 VLPs positive for EIA reactivity and GI.1-1968 as a negative control. The mean percent control binding was calculated by comparing the amount of VLP bound to Bi-HBGA in the presence of antibody pretreatment to the amount of VLP bound in the absence of antibody pretreatment. Error bars represent the standard errors of the means. (B) The Mean MAb concentration (μg/ml) needed to block 50% of GII.4 VLP ligand binding is indicated by the line in the graph. The upper and lower broken lines in the graph represent the maximum and minimum values. Asterisks indicate VLPs with BT50s significantly different from that of GII.4-2002.