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Detection of GII.4-2002 by Western blot analysis with anti-GII.4-2002 MAbs. GII.4-2002 VLPs were denatured, electrophoresed, and transferred to PVDF before being probed with 5 μg/ml and 1 μg/ml MAb. Mouse polyclonal anti-GII.4-2002 serum was used as a positive control for detection.