Table 2.
Summary of A*0101 and B*0702 iTopia and ELISpot data
| Allele | Peptide sequence | aab no. in Gag | % Peptide binding | Affinity (ED50) | Off-rate t1/2 (h) | Mean ELISpot responsec | Reported in HIV databasea |
|---|---|---|---|---|---|---|---|
| A*01:01 | NSSKVSQNY | 124–132 | 70.6 | 1.21 × 10−5 | 0.94 | 79 | Y |
| TTEGCQQIM | 53–61 | 26.2 | 1.70 × 10−5 | 0.33 | 77 | N | |
| YVDRFFKTL | 296–304 | 33.1 | 4.91 × 10−5 | 0.30 | 2,148 | Y | |
| SSSKVSQNY | 124–132 | 82.2 | 5.13 × 10−6 | 0.49 | 115 | N | |
| NSSQVSQNY | 124–132 | 73.9 | 5.02 × 10−6 | 0.48 | 96 | Y | |
| HSNQVSQNY | 124–132 | 69.9 | 1.43 × 10−5 | 0.31 | 115 | Y | |
| HSSQVSQNY | 124–132 | 83.7 | 4.30 × 10−6 | 0.79 | 74 | N | |
| NSSQVSRNY | 124–132 | 76.6 | 1.21 × 10−5 | 0.27 | 127 | N | |
| SSSQVSQNY | 124–132 | 85.1 | 5.18 × 10−6 | 0.43 | 130 | N | |
| B*07:02 | APPAEIFGM | 456–464 | 57.6 | 1.75 × 10−5 | 0.40 | 100 | N |
| APRKKGCWK | 406–414 | 145.3 | 8.96 E-07 | 0.78 | 148 | Y | |
| FPQSRPEPT | 447–455 | 55.6 | 1.50 × 10−5 | 0.70 | 55 | N | |
| GPGATLEEM | 338–346 | 35.1 | 1.78 × 10−5 | 0.81 | 80 | N | |
| GPGHKARVL | 355–363 | 93.1 | 4.80 × 10−6 | 0.42 | 84 | Y | |
| GPIPPGQMR | 221–229 | 45.9 | 4.84 × 10−4 | 0.78 | 215 | N | |
| GPSHKARVL | 355–363 | 91.6 | 3.14 × 10−5 | 0.44 | 95 | Y | |
| IVGGHQAAM | 190–198 | 46.4 | 4.49 × 10−5 | 0.47 | n/a | N | |
| IVQNAQGQM | 134–142 | 38.8 | 4.13 × 10−4 | 0.72 | n/a | N | |
| RALGPGATL | 335–343 | 52.2 | 1.49 × 10−5 | 0.45 | 115 | Y | |
| RLRPGGKKK | 20–28 | 32.4 | 7.83 × 10−5 | 0.71 | 135 | Y | |
| RPGNFPQSR | 443–451 | 37.3 | 5.37 × 10−5 | 0.73 | 80 | N | |
| SPRTLNAWV | 148–156 | 113.3 | 1.61 × 10−6 | 3.78 | 245 | Y | |
| TPQDLNMML | 180–188 | 36.9 | 8.12 × 10−5 | 0.83 | 905 | Y | |
| TPQEQIGWM | 242–250 | 44.8 | 2.60 × 10−5 | 0.67 | 185 | N | |
| TPQEQLGWM | 242–250 | 43.7 | 1.04 × 10−4 | 0.70 | <50 | N | |
| VRMYSPVSI | 274–282 | 41.6 | 1.69 × 10−4 | 0.83 | 435 | Y | |
| WPSSKGRPG | 437–445 | 81.0 | 2.71 × 10−5 | 0.40 | 115 | N | |
| YPLVSLKSL | 483–491 | 75.5 | 2.27 × 10−5 | 0.69 | 703 | N | |
| YVDRFFKTL | 296–304 | 60.0 | 4.37 × 10−5 | 0.50 | 1280 | Y | |
| KARVLAEAM | 359–367 | 66.5 | 8.08 × 10−6 | 0.44 | 78 | N | |
| QVQHTNIMM | 369–377 | 46.1 | 2.55 × 10−5 | 0.27 | 87 | N | |
| QAQQPNVMM | 369–377 | 46.2 | 1.92 × 10−5 | 0.35 | 96 | N | |
| QVNGNTAIM | 369–377 | 46.3 | 2.28 × 10−5 | 1.23 | 99 | N | |
| QANANTAIM | 369–377 | 46.0 | 1.36 × 10−5 | 1.35 | 109 | N | |
| ATNANAAIM | 370–378 | 37.4 | 2.08 × 10−5 | 0.25 | 95 | N | |
| NIMMQRGNF | 375–383 | 73.4 | 9.72 × 10−6 | 0.25 | 100 | N | |
| NIMMQRSNF | 375–383 | 73.0 | 9.31 × 10−6 | 0.25 | 121 | N | |
| NVMMQRSNF | 375–383 | 70.2 | 8.96 × 10−6 | 0.27 | 117 | N | |
| AIMMQRGNF | 375–383 | 91.6 | 3.45 × 10−6 | 0.38 | 113 | N |
a
Y, yes; N, no.
b
aa, amino acid.
c
n/a, not applicable.