Table 7.
Binding motif | Amino acid at indicated anchor position |
||||||||
---|---|---|---|---|---|---|---|---|---|
P1 | P2 | P3 | P4 | P5 | P6 | P7 | P8 | P9 | |
Previously defined motif | X | PVA | X | X | X | X | X | X | FLM(AIVYW) |
Epitopes identified in this study | A | P | P | A | E | I | F | G | M |
A | P | R | K | K | G | C | W | K | |
F | P | Q | S | R | P | E | P | T | |
G | P | G | A | T | L | E | E | M | |
G | P | G | H | K | A | R | V | L | |
G | P | I | P | P | G | Q | M | R | |
G | P | S | H | K | A | R | V | L | |
R | A | L | G | P | G | A | T | L | |
R | L | R | P | G | G | K | K | K | |
R | P | G | N | F | P | Q | S | R | |
S | P | R | T | L | N | A | W | V | |
T | P | Q | D | L | N | M | M | L | |
T | P | Q | E | Q | I | G | W | M | |
T | P | Q | E | Q | L | G | W | M | |
V | R | M | Y | S | P | V | S | I | |
W | P | S | S | K | G | R | P | G | |
Y | P | L | V | S | L | K | S | L | |
Y | V | D | R | F | F | K | T | L | |
G | P | G | H | K | A | R | V | L | |
G | P | S | H | K | A | R | V | L | |
K | A | R | V | L | A | E | A | M | |
Q | V | Q | H | T | N | I | M | M | |
Q | A | Q | Q | P | N | V | M | M | |
Q | V | N | G | N | T | A | I | M | |
Q | A | N | A | N | T | A | I | M | |
A | T | N | A | N | A | A | I | M | |
N | I | M | M | Q | R | G | N | F | |
N | I | M | M | Q | R | S | N | F | |
N | V | M | M | Q | R | S | N | F | |
A | I | M | M | Q | R | G | N | F |
Bolded amino acids are additional residues identified through iTopia peptide binding assays and confirmed by ELISpot assays. Previously described HLA binding motifs are summarized in reference 49.