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. 2012 Jan;86(2):1214–1226. doi: 10.1128/JVI.06189-11

Fig 1.

Fig 1

Putative epitopes A and D. (A) Amino acid differences between GII.4-1987 Camberwell and GII.4-2006 Minerva in the P2 region of VP1. Green shading indicates amino acids within putative epitope A, and orange indicates amino acids within putative epitope D. Shared residue 296S was included because it falls within putative epitope A. (B) Epitope A is shown in green, consisting of 6 amino acids at positions 294, 296, 297, 298, 368, and 372. Epitope D is shown in orange, consisting of 3 amino acids at positions 393 to 395. Black shading indicates the HBGA binding pocket. (C) Epitope A chimeric VLPs. The parental GII.4-2006 and GII.4-1987 amino acid residues are shown in purple and yellow, respectively. GII.4-2006 Minerva residues (purple) were replaced in each chimera by GII.4-1987 Camberwell residues at the positions shown in yellow. A total of four epitope A chimeric VLPs were created. (D) Representative EM images of parental and chimeric VLPs. (a) GII.4-1987; (b) GII.4-2006; (c) GII.4-1987(+06D); (d) GII.4-2006(+87D); (e) GII.4-2006(+87A.1); (f) GII.4-2006(+87A.2); (g) GII.4-2006(+87A.3); (h) GII.4-2006(+87A.4).