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. 2012 Jan;86(2):1214–1226. doi: 10.1128/JVI.06189-11

Fig 2.

Fig 2

Epitope D chimeric VLP reactivity with synthetic HBGAs and anti-GII MAbs. (A) HBGA reactivity. Synthetic CHO was diluted to 10 μg/ml and assayed by the CHO binding assay for interaction with GII.4-1987, GII.4-2006, GII.4-1987(+06D), and GII.4-2006(+87D). Amino acid residues from GII.4-1987 were exchanged with GII.4-2006 residues at positions 393 to 395. The dashed line represents 3 times the background level of binding and indicates a positive signal. Asterisks indicate significant differences. (B) Anti-GII.4 MAb reactivity. Anti-GII.4-1987 and -2006 MAbs were diluted to 2 μg/ml and assayed by EIA for reactivity with GII.4-1987, GII.4-2006, GII.4-1987(+06D), and GII.4-2006(+87D) VLPs. Amino acid residues from GII.4-1987 were exchanged with GII.4-2006 at positions 393 to 395. Bars represent the mean optical density values with standard errors of the means. The dashed line represents 3 times the background level of binding and indicates a positive signal.