Fig 3.

GII.4-2006(+87A.1) reactivity with anti-GII.4 MAbs. Anti-GII.4-1987 and -2006 IgG was diluted to 2 μg/ml and assayed by EIA for reactivity with GII.4-1987, GII.4-2006, and GII.4-2006(+87A.1) VLPs. Amino acid residues from GII.4-1987 were inserted into the GII.4-2006 backbone at positions 294, 297, 298, 368, and 372 to create GII.4-2006(+87A.1). Bars represent the mean optical density values with standard errors of the means. The dashed line represents 3 times the background level of binding and indicates a positive signal. Asterisks indicate significant differences.