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SEC and analysis by SDS-PAGE of P9-1 and P9-1ΔC. P9-1 and P9-1ΔC were purified by Ni-NTA affinity chromatography and then analyzed by SDS-PAGE and staining with Coomassie blue. Lane 1, purified P9-1; lane 2, purified P9-1ΔC. SEC was performed as described in the text. Solid line, purified P9-1; dotted line, purified P9-1ΔC. Each number with an arrow indicates the position and molecular mass of the respective marker protein (in kDa).
