Fig 8.
CAP expressed in trans inhibits the ability of wt 1a to stabilize and replicate BMV RNA. (A) Analysis of RNA3 levels in yeast expressing RNA3 alone or with wt 1a or with 1a and either CAP or HEL. (B) Positive-strand RNA3 and RNA4 from yeast expressing 2apol, RNA3, and 1a alone or 1a plus CAP or HEL. For panels A and B, total RNA was isolated from yeast cells and Northern blotting was used to examine the accumulation of RNA3 or RNA3 and RNA4 with a BMV RNA-specific probe. 18S rRNA was measured as a loading control. (C) Distribution of 1a, 1a fragments, and 2apol in membrane flotation gradient analyses of lysates from yeast cells coexpressing 2apol, 1a, and CAP or HEL. The flotation efficiency of each protein is shown on the right.