Fig 9.

CAP expressed in trans inhibits the ability of wt 1a to induce spherular structures. (A) Fluorescence microscopy images of cells coexpressing 1a-HA and CAP-GFP or 1a-HA and HEL-FLAG. PDI was used as an ER marker. Bars, 2 μm. (B) EM image of hexameric rings found between membrane layers in yeast coexpressing 1a and CAP. (C) Spherules 50 to 70 nm in diameter in yeast coexpressing 1a and HEL. (D and E) Anti-GFP (D) or anti-HA (E) immunogold EM labeling in yeast expressing CAP-GFP and 1a-HA. Nuc, nucleus; Cyto, cytoplasm. Bars, 200 nm (B and C) and 100 nm (D and E).