Fig 2.

Clonal entry kinetics of Sub07 subtype C isolates. Entry kinetics in a virus-cell BlaM fusion assay was assessed over 60 to 90 min in (A) U87-CD4-CCR5 cells, (B) TZM-bl cells, and (C) PBMC isolated from a single donor. Data were fitted to a one-phase association binding equation, Y = Y_max(1−e^−KX), and the time to half-maximal fusion (t_1/2max) was calculated for each experimental condition. Error bars represent the standard errors of the means of results from at least two experiments, each performed in triplicate. The goodness-of-fit measure (r²) was between 0.90 and 0.99 for all curve fits with the exception of Sub07res + 1 μM TAK-779 in PBMCs; for this condition, r² was 0.80.