Fig 4.

Unequivocal definition of novel HLA-B*57/58-restricted CD8⁺ T-cell epitopes. (A) HLA restriction of LW9 using peptide-pulsed or unpulsed partial effecter CTL HLA-matched B-cell lines sharing only 1 of the 6 HLA alleles of the R014 CTLs, as indicated on the y axis, in an intracellular cytokine-staining (ICS) assay. (B) Fine mapping of the LW9-Vif epitope using titrated peptide truncations of the predicted epitope in IFN-γ ELISPOT with an LW9-specific CTL line grown out from a chronically infected donor from Zimbabwe, R014 (HLA-A*3001/3301, B*4201/5703, and Cw*1701/1801), and used as effector input cells. (C) HLA-B*5703 tetramer staining of LW9 Vif-specific PBMCs from the same donor (subject R014) used for panels A and B.