Fig 8.
HLA-peptide affinity and HLA-peptide stability discriminate between immunogenic and nonimmunogenic optimal peptides and correlate with immunogenicity. (A and B) HLA-peptide binding affinities (A) and HLA-peptide stability (B) for immunogenic and nonimmunogenic peptides. Horizontal lines represent median values. (C and D) Binding affinity Kd values (nM) classified on a log scale (C) and peptide-HLA complex stability (h) (D) correlation with the percentage of individuals recognizing the corresponding OLP containing the optimal epitope, shown for groups of HLA-B*5702, HLA-B*5703, and HLA-B*5801 individuals as pooled data. Sloping lines represent Spearman rank correlations. The numbers in brackets refer to the number of individuals used for percent IFN-γ ELISPOT recognition of OLP. A Mann-Whitney U test was used for comparison of median values of immunogenic versus nonimmungenic peptides. P values of <0.05 were considered significant.